Cloned (Comment) | Organism |
---|---|
N-terminal fragment 1-318 and C-terminal fragment starting from position 397 seperately expressed in Escherichia coli BL21 | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-396 | no self adenylation activity, DNA-binding not affected | Geobacillus stearothermophilus |
DELTA319-670 | full self adenylation activity but strongly reduced DNA-binding and ligation activity | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | bound to the C-terminal fragment | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE, C-terminal fragment | Geobacillus stearothermophilus |
30040 | - |
ESI-mass spectrometry, C-terminal fragment | Geobacillus stearothermophilus |
35790 | - |
ESI-mass spectrometry, N-terminal fragment | Geobacillus stearothermophilus |
36000 | - |
SDS-PAGE, N-terminal fragment | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Geobacillus stearothermophilus | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
fragments overexpressed in Escherichia coli | Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-80°C, 10% glycerol | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Geobacillus stearothermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Geobacillus stearothermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Geobacillus stearothermophilus |